Fig 1: Random Forest Prediction Model demonstrating contributions of 4 ELISA protein concentrations and time of amniotic fluid sampling on the prediction of CDH. (A) Variable of importance (VIMP) plot. Variables with larger values are more important to the random forest model in predicting CDH. Boxplots present 95% confidence intervals (95% C.I.) of covariates’ contribution to the model. Covariates that contributed significantly, 95% C.I. did not include zero, are filled in purple and those with 95% C.I. included zero are blue. (B) Partial-dependence adjusted graph of association between SP-B and predicted likelihood of CDH, stratified by time of amniotic fluid sampling (Yellow—Early, Navy—Term). Adjusted CDH probability is based on random forest classification. Individual patients are denoted by the unfilled circles. (C) Partial-dependence adjusted graph of association between osteopontin and predicted likelihood of CDH, stratified by time of amniotic fluid sampling (Yellow—Early, Navy—Term). Adjusted CDH probability is based on random forest classification. (D) Unstratified partial-dependence adjusted graph of association between SP-B and predicted likelihood of CDH with model error detection labels. CDH population (squares) and healthy controls (triangles) are filled by model grouping assignment. Green indicates when the group is correctly categorized by the model, and red when incorrectly categorized. (E) Unstratified partial dependence-adjusted graph of association between osteopontin and predicted likelihood of CDH with model error detection labels. CDH population (squares) and healthy controls (triangles) are filled by model grouping assignment, green when the group is correctly categorized by the model, and red when incorrectly categorized. (F) Scatter plot of patients based on SP-B and osteopontin concentrations with model error detection labels. CDH population (squares) and healthy controls (triangles) are filled by model grouping assignment, green when the group is correctly categorized by the model, and red when incorrectly categorized.
Supplier Page from Novus Biologicals, a Bio-Techne Brand for Human SP-B/Surfactant Protein B ELISA Kit (Colorimetric)